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题名: Thf1 interacts with PS I and stabilizes the PS I complex in Synechococcus sp PCC7942
作者: Zhan, Jiao1; Zhu, Xi1, 2; Zhou, Wei1, 2; Chen, Hui1; He, Chenliu1; Wang, Qiang1
刊名: MOLECULAR MICROBIOLOGY
发表日期: 2016-11-01
DOI: 10.1111/mmi.13488
卷: 102, 期:4, 页:738-751
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Microbiology
英文摘要: Thylakoid formation1 protein (Thf1) is a multifunctional protein that is conserved in all photosynthetic organisms. In this study, we used the model cyanobacterium Synechococcus sp. PCC7942 (hereafter Synechococcus) to show that the level of Thf1 is altered in response to various stress conditions. Although this protein has been reported to be involved in thylakoid formation, the thylakoid membrane in the thf1 deletion strain (Thf1) was not affected. Compared with the WT, Thf1 showed reduced PS II activity, with increased levels of D1 under high light (HL) conditions, which was resulted from blocked D1 degradation by the FtsH protease and thus inhibits PS II repair. PS I was found to be more seriously affected than PS II in Thf1, even under low light conditions, suggesting that PS I damage could be the primary effect of thf1 deletion in Synechococcus. Further analysis revealed that the Thf1 mutant had a lower PS I subunit content and lower PS I stability under HL conditions. Further sucrose gradient fractionation of the membrane protein complexes and crosslinking and immunoblot analysis indicated that Thf1 interacts with PS I. Together, our results reveal that Thf1 interacts with PS I and thereby stabilizes PS I in Synechococcus.
关键词[WOS]: SP PCC 6803 ; SECONDARY STRUCTURE PREDICTION ; SP STRAIN PCC-6803 ; PHOTOSYSTEM-I ; THYLAKOID MEMBRANES ; CYANOBACTERIAL YCF37 ; CRYSTAL-STRUCTURE ; LEAF VARIEGATION ; PROTEIN ; SYNECHOCYSTIS
语种: 英语
项目资助者: National Program on Key Basic Research Project(2012CB224803) ; National Natural Science Foundation of China(31270094)
WOS记录号: WOS:000387757100012
ISSN号: 0950-382X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28665
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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