Science & Technology
; Life Sciences & Biomedicine
The underlying mechanism of polybrominated diphenyl ether (PBDE)-induced neurotoxicity is still a major concern due to its ubiquitous nature and persistence. Here, zebrafish embryos (2h postfertilization, hpf) were exposed to different concentrations of the commercial PBDE mixture DE-71 (0-100 mu gl(-1)) until 120 hpf, and the impact on neural development and serotonergic system was investigated. The in vivo results revealed significantly reduced transcription of genes involved in neurogenesis (fgf8, shha, wnt1), and contents of proteins in neuronal morphogenesis (myelin basic protein, synapsin IIa), suggesting an impairment of neural development in zebrafish embryos. Further results demonstrated a reduction of 5-hydroxytryptamine neuron and a dose-dependent decrease of whole-body serotonin levels, as well as the transcription of genes involved in serotonergic synthesis (tph1, tph2, trhr) and neurotransmission (serta/b, htr1aa/b). In addition, we predicted possible targets of PBDEs by molecular docking, and the results indicated that PBDE congeners showed high binding affinities with fibroblast growth factor 8 other than SHH and HTR1B. Taken together, this study demonstrated that PBDE exposure during embryogenesis could damage neural development and cause impairment of the serotonergic system as secondary effects in the zebrafish larvae. Copyright (c) 2016 John Wiley & Sons, Ltd.
National Natural Science Foundation of China(21307153
; National Environmental Protection Public Welfare Science and Technology Research Program of China(201309047)
; Knowledge Innovation Program of the Chinese Academy of Sciences(Y25E03)
1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China 4.Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China