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题名: Microcystin-Bound Protein Patterns in Different Cultures of Microcystis aeruginosa and Field Samples
作者: Wei, Nian1, 2; Hu, Lili1, 2; Song, Lirong1; Gan, Nanqin1
关键词: microcystin ; microcystin-bound protein ; batch culture ; semi-continuous culture ; Microcystis aeruginosa ; Lake Taihu
刊名: TOXINS
发表日期: 2016-10-01
DOI: 10.3390/toxins8100293
卷: 8, 期:10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要: Micocystin (MC) exists in Microcystis cells in two different forms, free and protein-bound. We examined the dynamic change in extracellular free MCs, intracellular free MCs and protein-bound MCs in both batch cultures and semi-continuous cultures, using high performance liquid chromatography and Western blot. The results showed that the free MC per cell remained constant, while the quantity of protein-bound MCs increased with the growth of Microcystis cells in both kinds of culture. Significant changes in the dominant MC-bound proteins occurred in the late exponential growth phase of batch cultures, while the dominant MC-bound proteins in semi-continuous cultures remained the same. In field samples collected at different months in Lake Taihu, the dominant MC-bound proteins were shown to be similar, but the amount of protein-bound MC varied and correlated with the intracellular MC content. We identified MC-bound proteins by two-dimensional electrophoresis immunoblots and mass spectrometry. The 60 kDa chaperonin GroEL was a prominent MC-bound protein. Three essential glycolytic enzymes and ATP synthase alpha subunit were also major targets of MC-binding, which might contribute to sustained growth in semi-continuous culture. Our results indicate that protein-bound MC may be important for sustaining growth and adaptation of Microcystis sp.
关键词[WOS]: BLOOM-FORMING MICROCYSTIS ; CYANOBACTERIAL TOXINS ; MICROGRAM QUANTITIES ; HIGH LIGHT ; PCC-7806 ; DYNAMICS ; LAKE ; LR ; PHOSPHATASE-2A ; IDENTIFICATION
语种: 英语
项目资助者: National Natural Science Foundation of China(31370418 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ07) ; 41561144008)
WOS记录号: WOS:000387347300024
ISSN号: 2072-6651
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28684
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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