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题名: Comparative Analysis of Ciliary Membranes and Ectosomes
作者: Long, Huan1; Zhang, Fan1, 2; Xu, Nannan1, 2; Liu, Gai1; Diener, Dennis R.3; Rosenbaum, Joel L.3; Huang, Kaiyao1
刊名: CURRENT BIOLOGY
发表日期: 2016-12-19
DOI: 10.1016/j.cub.2016.09.055
卷: 26, 期:24, 页:3327-3335
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
英文摘要: Primary and motile cilia/flagella function as cellular antennae, receiving signals from the environment and subsequently activating signaling pathways that are critical for cellular homeostasis and differentiation [1-3]. Recent work with the green alga Chlamydomonas and the nematode C. elegans demonstrated that ectosomes can be released from the cilium and can mediate the intercellular communication [4-9]. To better understand the function of flagellar ectosomes, we have compared their protein composition to that of the flagellar membrane from which they are derived. Ectosomes released from flagella have a unique protein composition, being enriched in a subset of flagellar membrane proteins, proteases, proteins from the endosomal sorting complex required for transport (ESCRT) [10-12], small GTPases, and ubiquitinated proteins. Live imaging showed that an ESCRT-related protein (PDCD6) was enriched in ectosomes released from flagella during gamete activation. We devised a sensitive and rapid assay to monitor ectosome release using luciferase fused to PDCD6 and a mutated ubiquitin. Ectosome release increased when cells underwent flagellar resorption. Knockdown of two ESCRT-related proteins, PDCD6 and VPS4, attenuated ectosome release during flagellar shortening and shortening was slowed. These data suggest that the ESCRT proteins mediate ectosome release and thereby influence flagellar shortening in Chlamydomonas. In addition, the prevalence of receptors such as agglutinin and ubiquitinated proteins in ciliary ectosomes suggests that they are involved in cell signaling and turnover of ciliary proteins.
关键词[WOS]: EXTRACELLULAR VESICLES ; CHLAMYDOMONAS-REINHARDTII ; ESCRT PATHWAY ; FLAGELLA ; ALIX ; BIOGENESIS ; PROTEINS ; EXOSOMES ; REVEALS ; CILIUM
语种: 英语
项目资助者: National Nature Science Foundation of China(31171287 ; NIH(GM014642) ; 31401154)
WOS记录号: WOS:000390666200026
ISSN号: 0960-9822
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28759
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
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